Review

rabbit anti furin pabs (Proteintech)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech rabbit anti furin pabs
Rabbit Anti Furin Pabs, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+furin/FURIN+Antibody/pmc12456011-181-0-6
Average 93 stars, based on 34 article reviews

rabbit anti furin pabs - by Bioz Stars, 2026-10

93/100 stars

Images

other:

Article Title: Furin inhibitor D6R suppresses epithelial-mesenchymal transition in SW1990 and PaTu8988 cells via the Hippo-YAP signaling pathway
Article Snippet: Primary antibodies included; rabbit anti-Furin (1:500; cat no. 18413-1-AP; Proteintech Group, Inc., Chicago, IL, USA) and mouse anti-β-Tubulin (1:10,000, cat no. 6181), rabbit anti-N-Cadherin (1:1,000; cat no. 13116), rabbit anti-E-Cadherin (1:1,000; cat no. 3195), rabbit anti-Vimentin (1:1,000; cat no. 5741), rabbit anti-DBF2 kinase activator protein MOB1 (Mob1; 1:1,000, cat no. 13730), rabbit anti-p-Mob1 (1:1,000, cat no. 8699), rabbit anti-yes-associated protein (YAP; 1:1,000; cat no. 8418), rabbit anti-p-YAP (1:1,000, cat no. 13619) (all purchased from Cell Signaling Technology, Inc., Danvers, MA, USA).

Article Title: Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination.
Article Snippet: Antibodies used include: Horseradish peroxidase (HRP)-conjugated anti-FLAG (Sigma, A8592, 1:8000), HRP-conjugated anti-HA (Sigma, H6533, 1:5000), HRP-conjugated anti-Actin (Proteintech, HRP-60008, 1:10 000), HRP-conjugated anti-His (Proteintech, HRP-66005, 1:8000), rabbit anti-furin (Proteintech,18413-1-AP, 1:5000), mouse anti-Myc (Cell Signaling, 2276S, 1:2000), rabbit anti-SARS-CoV-2 (Sino Biological, 40590- T62, 1:8000), mouse anti-MERS-CoV (Sino Biological, 40070-MM11, 1:5000), mouse anti-USP32 A-10 (Santa Cruz Biotechnology, SC-374465, 1:2000), and rabbit anti-MARCHF2 (Abmart, PS07245S, 1:200).

Article Title: Furin promotes epithelial-mesenchymal transition in pancreatic cancer cells via Hippo-YAP pathway.
Article Snippet: The antibodies were rabbit anti-Furin (18413-1-AP; Proteintech, Rosemont, IL, USA), mouse anti-β-Tubulin (cat. no. 6181; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Flag (cat. no. F1804; Sigma-Aldrich), rabbit anti-N-cadherin (cat. no. 13116; Cell Signaling Technology), rabbit anti-E-cadherin (cat. no. 3195; Cell Signaling Technology), rabbit anti-Vimentin (cat. no. 5741; Cell Signaling Technology), rabbit anti-YAP (cat. no. 8418; Cell Signaling Technology), rabbit anti-p-YAP (cat. no. 13619; Cell Signaling Technology), rabbit anti-Mob1 (cat. no. 13730; Cell Signaling Technology), rabbit anti-p-Mob1 (cat. no. 8699; Cell Signaling Technology).

Incubation:

Article Title: Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial–mesenchymal transition in pancreatic cancer cells
Article Snippet: .. The blots were incubated with primary antibodies rabbit anti-MeCP2 (Cell Signaling, CAT 3456), rabbit anti-Furin (Proteintech, CAT 18413-1-AP), anti-TGF-β1 (Cell Signaling, CAT 3709), anti-TGF-βR (Cell Signaling, CAT 3712), anti-MMP2 (Immunoway, CAT YT2798), anti-MMP9 (Immunoway, CAT YT1892), EMT Kit (Cell Signaling, CAT 9782), Smad2/3 Antibody Sampler Kit (Cell Signaling, CAT 12747), mouse anti-Flag (SIGMA, CAT 6631), mouse anti-β-Tubulin (Cell Signaling, CAT4466). ..

Image Search Results

Upregulation of the UII system in the kidneys of neonatal pigs following IR injury. Quantitative RT-PCR analysis showing relative mRNA expression levels of (A) furin, (B) UII, (C) URP, and (D) UT in whole kidney tissue, and (E) UT in intrarenal arteries from sham and IR piglets ( n = 4 per group). (F–G) Representative Western blot and densitometric analysis of UT protein expression in intrarenal arteries ( n = 4 per group). (H–I) Western blot and quantification of furin protein expression in kidney tissues ( n = 4 per group). (J–K) representative immunohistochemistry (IHC) images and quantification of UII immunostaining intensity in renal tubules from sham and IR piglets (4 kidney sections per group). * p < 0.05, unpaired t -test.

Journal: Renal Failure

Article Title: Urotensin II system contributes to ischemic acute kidney injury in neonatal pigs

doi: 10.1080/0886022X.2025.2534018

Figure Lengend Snippet: Upregulation of the UII system in the kidneys of neonatal pigs following IR injury. Quantitative RT-PCR analysis showing relative mRNA expression levels of (A) furin, (B) UII, (C) URP, and (D) UT in whole kidney tissue, and (E) UT in intrarenal arteries from sham and IR piglets ( n = 4 per group). (F–G) Representative Western blot and densitometric analysis of UT protein expression in intrarenal arteries ( n = 4 per group). (H–I) Western blot and quantification of furin protein expression in kidney tissues ( n = 4 per group). (J–K) representative immunohistochemistry (IHC) images and quantification of UII immunostaining intensity in renal tubules from sham and IR piglets (4 kidney sections per group). * p < 0.05, unpaired t -test.

Article Snippet: For Western blot analysis, the primary antibodies included a rabbit polyclonal anti-furin antibody (18413-1-AP, Proteintech, Rosemont, IL, USA) at a 1:500 dilution and a rabbit polyclonal anti-GPR14 (UT receptor) antibody (TA358466, OriGene Technologies, Rockville, MD, USA) at a 1:100 dilution.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemistry, Immunostaining

Furin inhibition reduces cIR-driven UII production, and UT and furin blockade attenuate cIR-induced cytotoxicity in primary neonatal pig proximal tubule epithelial cells. (A) Representative fluorescence microscopy images showing furin and UII immunostaining and their colocalization in primary neonatal pig proximal tubule epithelial cells (PTECs; 3 biological replicates). (B) Quantification of intracellular furin levels in control (ctrl) and chemical ischemia-reperfusion (cIR)–treated cells ( n = 4). (C) Secreted UII levels in cIR-treated cells in the absence or presence of the furin inhibitor SSM3 trifluoroacetate (SSM3; n = 4). (D) cIR-induced cytotoxicity was significantly reduced by pharmacological inhibition of furin (SSM3) and by UT inhibition with urantide (URTD). * p < 0.05 ctrl vs. cIR; p < 0.05 cIR vs. SSM3 + cIR; $ p < 0.05 cIR vs. both SSM3 + cIR and URTD + cIR. Unpaired t -test (B); One-way ANOVA and Holm-Šídák’s multiple comparisons tests (C–D); n = 4, each. Scale bar = 50 µm.

Journal: Renal Failure

Article Title: Urotensin II system contributes to ischemic acute kidney injury in neonatal pigs

doi: 10.1080/0886022X.2025.2534018

Figure Lengend Snippet: Furin inhibition reduces cIR-driven UII production, and UT and furin blockade attenuate cIR-induced cytotoxicity in primary neonatal pig proximal tubule epithelial cells. (A) Representative fluorescence microscopy images showing furin and UII immunostaining and their colocalization in primary neonatal pig proximal tubule epithelial cells (PTECs; 3 biological replicates). (B) Quantification of intracellular furin levels in control (ctrl) and chemical ischemia-reperfusion (cIR)–treated cells ( n = 4). (C) Secreted UII levels in cIR-treated cells in the absence or presence of the furin inhibitor SSM3 trifluoroacetate (SSM3; n = 4). (D) cIR-induced cytotoxicity was significantly reduced by pharmacological inhibition of furin (SSM3) and by UT inhibition with urantide (URTD). * p < 0.05 ctrl vs. cIR; p < 0.05 cIR vs. SSM3 + cIR; $ p < 0.05 cIR vs. both SSM3 + cIR and URTD + cIR. Unpaired t -test (B); One-way ANOVA and Holm-Šídák’s multiple comparisons tests (C–D); n = 4, each. Scale bar = 50 µm.

Techniques: Inhibition, Fluorescence, Microscopy, Immunostaining, Control

List of the oligonucleotides used for the host gene and cytokine expression profiling.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: List of the oligonucleotides used for the host gene and cytokine expression profiling.

Article Snippet: Furin rabbit anti-bovine polyclonal (Cat. No. ARP45328_P050), ACE2 rabbit anti-bovine polyclonal (Cat. No. ARP53751_P050), TMPRSS2 rabbit anti-bovine polyclonal (Cat. No. ARP46628_P050), and anti-NRP1 rabbit anti-bovine polyclonal (Cat. No. ARP59101_P050) were purchased from Aviva Systems Biology.

Techniques: Expressing

The host Furin as a potential novel target for the bovine miRNA16a. ( A ) In silico prediction of miRNA16a targeting the 3’ UTR of the host cell Furin. The context + + score percentile represents the binding energy of the miRNA with the target gene. ( B ) Multiple sequence alignment shows that the miRNA16a/Furin binding region (indicated in the red color font boxes) is conserved among eight species, including humans, mice, and bovines. ( C ) The MDBK cells were transfected with either miR-Scr or miRNA16a, followed by BCoV/Ent or BCoV/Resp infection. After 72 h, the samples were subjected to qRT‒PCR. ( D ) Western blot analysis of host Furin protein expression in the MDBK cells in the miR-Scr- and miRNA16a-transfected groups. ( E ) Western blot band density of Furin protein normalized to that of β-actin in the MDBK cells. ( F ) The BEC cells were transfected with scrambled and miRNA16a, followed by BCoV enteric and respiratory isolate infection. After 72 h, samples were collected for the qRT-PCR analysis to assess the mRNA expression level of the host Furin in both the scrambled- and miRNA16a-transfected groups. ( G ) Western blot analysis of host Furin protein expression in the scrambled- and miRNA16aa-transfected groups of BEC cells. ( H ) Western blot band density of Furin protein normalized to that of β-actin in BECs. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: The host Furin as a potential novel target for the bovine miRNA16a. ( A ) In silico prediction of miRNA16a targeting the 3’ UTR of the host cell Furin. The context + + score percentile represents the binding energy of the miRNA with the target gene. ( B ) Multiple sequence alignment shows that the miRNA16a/Furin binding region (indicated in the red color font boxes) is conserved among eight species, including humans, mice, and bovines. ( C ) The MDBK cells were transfected with either miR-Scr or miRNA16a, followed by BCoV/Ent or BCoV/Resp infection. After 72 h, the samples were subjected to qRT‒PCR. ( D ) Western blot analysis of host Furin protein expression in the MDBK cells in the miR-Scr- and miRNA16a-transfected groups. ( E ) Western blot band density of Furin protein normalized to that of β-actin in the MDBK cells. ( F ) The BEC cells were transfected with scrambled and miRNA16a, followed by BCoV enteric and respiratory isolate infection. After 72 h, samples were collected for the qRT-PCR analysis to assess the mRNA expression level of the host Furin in both the scrambled- and miRNA16a-transfected groups. ( G ) Western blot analysis of host Furin protein expression in the scrambled- and miRNA16aa-transfected groups of BEC cells. ( H ) Western blot band density of Furin protein normalized to that of β-actin in BECs. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Techniques: In Silico, Binding Assay, Sequencing, Transfection, Infection, Western Blot, Expressing, Quantitative RT-PCR, Comparison

Validation of the host cell furin as a functional target for the host cell miRNA16a. ( A ) The MDBK cells were transfected independently with either miR-Scr or Antagomir-16a, followed by BCoV/Ent or BCoV/Resp infection. After 72 h of transfection, the collected samples were subjected to qRT-PCR to evaluate the host cell furin mRNA expression levels. ( B ) Western blot analysis of host Furin protein and β-actin expression in the MDBK cells in the miR-Scr- and Antagomir-16a-transfected groups. ( C ) Western blot band density of Furin protein normalized to that of β-actin in the MDBK cells. ( D ) The results of the dual-luciferase reporter assay. HEK cells were transfected with different combinations of the Furin 3’UTR (WT) or mutant (Mut) plasmid or vector only (pmir-GLo) with miRNA16a, Antagomir-16a, and miR-Scr, as indicated. The dual luciferase assay was conducted, and the relative luciferase activity was calculated using red firefly luciferase signals as a normalization control for the green Renilla luciferase signals. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: Validation of the host cell furin as a functional target for the host cell miRNA16a. ( A ) The MDBK cells were transfected independently with either miR-Scr or Antagomir-16a, followed by BCoV/Ent or BCoV/Resp infection. After 72 h of transfection, the collected samples were subjected to qRT-PCR to evaluate the host cell furin mRNA expression levels. ( B ) Western blot analysis of host Furin protein and β-actin expression in the MDBK cells in the miR-Scr- and Antagomir-16a-transfected groups. ( C ) Western blot band density of Furin protein normalized to that of β-actin in the MDBK cells. ( D ) The results of the dual-luciferase reporter assay. HEK cells were transfected with different combinations of the Furin 3’UTR (WT) or mutant (Mut) plasmid or vector only (pmir-GLo) with miRNA16a, Antagomir-16a, and miR-Scr, as indicated. The dual luciferase assay was conducted, and the relative luciferase activity was calculated using red firefly luciferase signals as a normalization control for the green Renilla luciferase signals. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Techniques: Biomarker Discovery, Functional Assay, Transfection, Infection, Quantitative RT-PCR, Expressing, Western Blot, Luciferase, Reporter Assay, Mutagenesis, Plasmid Preparation, Activity Assay, Control, Comparison

The impacts of silencing the host Furin and BCoV-S glycoproteins by specifically designed siRNA molecules on the BCoV replication. ( A ) Schematic representation of the siRNA transfection BCoV infection experiments: the transfection process of the scrambled-siRNA, siRNA-Furin, and the siRNA-BCoV-S glycoproteins into the MDBK the BEC cells was illustrated, followed by infection with independent BCoV/Ent or BCoV/Resp isolates infection in these cells for 72 h and subsequent sample collection for further analysis. ( B ) The MDBK cells were transfected with 50 ng or 100 ng of the siRNA-Furin, followed by western blot analysis of Furin protein expression levels. ( C ) Western blot band density of the Furin protein normalized to that of β-actin in the MDBK cells. ( D ) The MDBK cells were transfected with 50 ng or 100 ng of siRNA BCoV/-S, followed by western blot analysis of BCoV/S protein expression. ( E ) Results of the western blot band density of the BCoV/S glycoprotein normalized to that of β-actin in the MDBK cells. ( F ) The qRT-PCR results show the Furin mRNA expression levels in the scrambled siRNA- and the siRNA-Furin-transfected MDBK cells ( G ) and BECs. ( H ) The qRT-PCR results analysis shows the genome copy numbers of the BCoV in the cases of the scrambled-siRNA and the siRNA-Furin-transfected MDBK cells ( I ) and BEC cells. ( J ) The qRT-PCR analysis results show the genome copy numbers of the BCoV in the case of the scrambled-siRNA and siRNA-BCoV/Spike-transfected MDBK cells ( K ) and the BEC cells. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: The impacts of silencing the host Furin and BCoV-S glycoproteins by specifically designed siRNA molecules on the BCoV replication. ( A ) Schematic representation of the siRNA transfection BCoV infection experiments: the transfection process of the scrambled-siRNA, siRNA-Furin, and the siRNA-BCoV-S glycoproteins into the MDBK the BEC cells was illustrated, followed by infection with independent BCoV/Ent or BCoV/Resp isolates infection in these cells for 72 h and subsequent sample collection for further analysis. ( B ) The MDBK cells were transfected with 50 ng or 100 ng of the siRNA-Furin, followed by western blot analysis of Furin protein expression levels. ( C ) Western blot band density of the Furin protein normalized to that of β-actin in the MDBK cells. ( D ) The MDBK cells were transfected with 50 ng or 100 ng of siRNA BCoV/-S, followed by western blot analysis of BCoV/S protein expression. ( E ) Results of the western blot band density of the BCoV/S glycoprotein normalized to that of β-actin in the MDBK cells. ( F ) The qRT-PCR results show the Furin mRNA expression levels in the scrambled siRNA- and the siRNA-Furin-transfected MDBK cells ( G ) and BECs. ( H ) The qRT-PCR results analysis shows the genome copy numbers of the BCoV in the cases of the scrambled-siRNA and the siRNA-Furin-transfected MDBK cells ( I ) and BEC cells. ( J ) The qRT-PCR analysis results show the genome copy numbers of the BCoV in the case of the scrambled-siRNA and siRNA-BCoV/Spike-transfected MDBK cells ( K ) and the BEC cells. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Techniques: Transfection, Infection, Western Blot, Expressing, Quantitative RT-PCR, Comparison

Silencing of the host cell Furin protein and the BCoV-S glycoprotein negatively impacted BCoV infectivity in bovine cells. ( A ) The viral infectivity of the BCoV/Ent or BCoV/Resp was markedly inhibited in cells transfected with siRNA-BCoV/S or (B) the siRNA-Furin, as measured by the viral plaque assay. The plaque-forming units (PFUs) were used to determine the viral titer in each virus-infected group. ( C ) The MDBK cells were transfected with scrambled siRNA (Scr-siRNA), siRNA-Furin, or siRNA BCoV/-Spike, followed by infection with either the BCoV/Ent or the BCoV/Resp. Cells were lysed after 72 hpi, and western blot analysis was performed to observe the protein expression of the BCoV-S, the BCoV-N, and the host cell Furin proteins expression. ( D ) Western blot band density of the Furin protein, ( E ) BCoV spike protein, and ( F ) BCoV-N protein was normalized to that of β-actin in the MDBK cells. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, *** p < 0.001, and **** p < 0.0001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: Silencing of the host cell Furin protein and the BCoV-S glycoprotein negatively impacted BCoV infectivity in bovine cells. ( A ) The viral infectivity of the BCoV/Ent or BCoV/Resp was markedly inhibited in cells transfected with siRNA-BCoV/S or (B) the siRNA-Furin, as measured by the viral plaque assay. The plaque-forming units (PFUs) were used to determine the viral titer in each virus-infected group. ( C ) The MDBK cells were transfected with scrambled siRNA (Scr-siRNA), siRNA-Furin, or siRNA BCoV/-Spike, followed by infection with either the BCoV/Ent or the BCoV/Resp. Cells were lysed after 72 hpi, and western blot analysis was performed to observe the protein expression of the BCoV-S, the BCoV-N, and the host cell Furin proteins expression. ( D ) Western blot band density of the Furin protein, ( E ) BCoV spike protein, and ( F ) BCoV-N protein was normalized to that of β-actin in the MDBK cells. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, *** p < 0.001, and **** p < 0.0001.

Techniques: Infection, Transfection, Viral Plaque Assay, Virus, Western Blot, Expressing, Comparison

Silencing of the host cell Furin and BCoV-S glycoproteins impacted the expression levels of some other coronavirus replication-related proteins (ACE2, NRP1, and TMPRSS2) which further impacted BCoV/Ent/BCoV/Resp replication. ( A ) The MDBK cells were transfected with Scr-siRNA control, siRNA-Furin, or siRNA BCoV/Spike glycoprotein, followed by the infection with either BCoV/Ent or BCoV/Resp. Cell lysates were collected after 72 hpi and subjected to Western blot analysis to assess the protein expression levels of the bovine (ACE2, NRP1, TMPRSS2, and bovine β-actin) proteins. ( B ) Results of the qRT-PCR analysis of bovine TMPRSS2 mRNA expression levels in the case of the siRNA-Scrambled-, the siRNA-Furin-, or the siRNA-BCoV-S transfected MDBK cells. ( C ) Results of the analysis of the western blot band densities of the TMPRSS2 protein expression normalized to that of bovine β-actin protein. ( D ) Results of the qRT-PCR analysis of bovine ACE2 mRNA expression levels. ( E ) Results of the western blot band densities of the ACE2 protein normalized to that of β-actin. ( F ) Results of the qRT-PCR analysis of bovine NRP1 mRNA expression. ( G ) Results of the western blot band densities of the NRP1 protein expression levels normalized to that of β-actin. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: Silencing of the host cell Furin and BCoV-S glycoproteins impacted the expression levels of some other coronavirus replication-related proteins (ACE2, NRP1, and TMPRSS2) which further impacted BCoV/Ent/BCoV/Resp replication. ( A ) The MDBK cells were transfected with Scr-siRNA control, siRNA-Furin, or siRNA BCoV/Spike glycoprotein, followed by the infection with either BCoV/Ent or BCoV/Resp. Cell lysates were collected after 72 hpi and subjected to Western blot analysis to assess the protein expression levels of the bovine (ACE2, NRP1, TMPRSS2, and bovine β-actin) proteins. ( B ) Results of the qRT-PCR analysis of bovine TMPRSS2 mRNA expression levels in the case of the siRNA-Scrambled-, the siRNA-Furin-, or the siRNA-BCoV-S transfected MDBK cells. ( C ) Results of the analysis of the western blot band densities of the TMPRSS2 protein expression normalized to that of bovine β-actin protein. ( D ) Results of the qRT-PCR analysis of bovine ACE2 mRNA expression levels. ( E ) Results of the western blot band densities of the ACE2 protein normalized to that of β-actin. ( F ) Results of the qRT-PCR analysis of bovine NRP1 mRNA expression. ( G ) Results of the western blot band densities of the NRP1 protein expression levels normalized to that of β-actin. The data of all the experiments represent at least three biological repeats. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Techniques: Expressing, Transfection, Control, Infection, Western Blot, Quantitative RT-PCR, Comparison

Activation of the host cell cytokine-like Strome upon miRNA16a overexpression during BCoV infection in bovine cells. The MDBK cells were transfected with either Scr-miRNA or miRNA16a, followed by infection with BCoV/Ent or BCoV/Resp. The results. Results of the qRT-PCR analysis to quantify the mRNA expression of ( A ) IFN-α; ( B ) IFN-β; ( C ) IFN-γ; ( D ) IL-6; ( E ) and IL-10. ( F ) The MDBK cells were transfected with Scr-siRNA control, siRNA-Furin, or siRNA-BCoV-Spike, followed by BCoV/Ent or BCoV/Resp infection. The qRT-PCR analysis of the mRNA expression levels of IFN-α, ( G ) IFN-β, ( H ) IFN-γ, ( I ) IL-6, ( J ) and IL-10. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: Activation of the host cell cytokine-like Strome upon miRNA16a overexpression during BCoV infection in bovine cells. The MDBK cells were transfected with either Scr-miRNA or miRNA16a, followed by infection with BCoV/Ent or BCoV/Resp. The results. Results of the qRT-PCR analysis to quantify the mRNA expression of ( A ) IFN-α; ( B ) IFN-β; ( C ) IFN-γ; ( D ) IL-6; ( E ) and IL-10. ( F ) The MDBK cells were transfected with Scr-siRNA control, siRNA-Furin, or siRNA-BCoV-Spike, followed by BCoV/Ent or BCoV/Resp infection. The qRT-PCR analysis of the mRNA expression levels of IFN-α, ( G ) IFN-β, ( H ) IFN-γ, ( I ) IL-6, ( J ) and IL-10. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Techniques: Activation Assay, Over Expression, Infection, Transfection, Quantitative RT-PCR, Expressing, Control, Comparison

The proposed model of the mechanism of the dual actions of the host cell miRNA16a in restricting BCoV replication through targeting the BCoV-S glycoprotein and the host cell Furin and enhancing cytokine expression. ( A ) Schematic representation of the BCoV-Spike protein, highlighting the S1 and S2 subunits and Furin cleavage site (S1/S2). The miRNA16a targets two regions within the S1 subunit, indicated in red (starting positions 24,762 and 24,928). ( B ) A schematic diagram of the bovine Furin shows the miRNA16a target sites in the 3’UTR of the bovine host cell furin; red fonts indicate the starting nucleotides of the seed region of miRNA16a (starting position 793). ( C ) Illustration of the bovine cells transfected with miRNA scrambled (miRNA-Scr) followed by BCoV infection. The BCoV viral genome is released into the cytoplasm of infected cells, after which viral replication and the production of nested sets of sg mRNAs are initiated. BCoV infection markedly inhibited the expression of some host cytokines (IFN-α, IFN-β, IFN-γ, IL-6, and IL-10) but enhanced BCoV production. ( D ) A schematic illustration of the bovine cells transfected with miRNA16a followed by infection with BCoV. The miRNA16a targets host Furin at the cell surface, reducing the BCoV replication by abolishing the BCoV/S glycoprotein cleavage. The overexpression of miRNA16a also showed marked inhibition of the BCoV-S glycoprotein expression levels, ultimately reducing the production of other viral proteins, particularly BCoV-N. The miRNA16a, which targets BCoV-S and the host Furin, substantially inhibited the production of new BCoV progeny. Moreover, this targeting markedly increased the expression of some host cell cytokines, especially the (IFN-α, IFN-β, IFN-γ, IL-6, and IL-10), which led to further inhibition of BCoV progeny release.

Journal: Scientific Reports

Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

doi: 10.1038/s41598-024-80708-4

Figure Lengend Snippet: The proposed model of the mechanism of the dual actions of the host cell miRNA16a in restricting BCoV replication through targeting the BCoV-S glycoprotein and the host cell Furin and enhancing cytokine expression. ( A ) Schematic representation of the BCoV-Spike protein, highlighting the S1 and S2 subunits and Furin cleavage site (S1/S2). The miRNA16a targets two regions within the S1 subunit, indicated in red (starting positions 24,762 and 24,928). ( B ) A schematic diagram of the bovine Furin shows the miRNA16a target sites in the 3’UTR of the bovine host cell furin; red fonts indicate the starting nucleotides of the seed region of miRNA16a (starting position 793). ( C ) Illustration of the bovine cells transfected with miRNA scrambled (miRNA-Scr) followed by BCoV infection. The BCoV viral genome is released into the cytoplasm of infected cells, after which viral replication and the production of nested sets of sg mRNAs are initiated. BCoV infection markedly inhibited the expression of some host cytokines (IFN-α, IFN-β, IFN-γ, IL-6, and IL-10) but enhanced BCoV production. ( D ) A schematic illustration of the bovine cells transfected with miRNA16a followed by infection with BCoV. The miRNA16a targets host Furin at the cell surface, reducing the BCoV replication by abolishing the BCoV/S glycoprotein cleavage. The overexpression of miRNA16a also showed marked inhibition of the BCoV-S glycoprotein expression levels, ultimately reducing the production of other viral proteins, particularly BCoV-N. The miRNA16a, which targets BCoV-S and the host Furin, substantially inhibited the production of new BCoV progeny. Moreover, this targeting markedly increased the expression of some host cell cytokines, especially the (IFN-α, IFN-β, IFN-γ, IL-6, and IL-10), which led to further inhibition of BCoV progeny release.

Techniques: Expressing, Transfection, Infection, Over Expression, Inhibition

Review

rabbit anti furin pabs (Proteintech)

Structured Review

Images

Related Articles

other:

Incubation:

Similar Products

Image Search Results